Methods

·        Flow Cytometry

·        Protein isolation, -quantification and Western Blot

·        Tissue embedding (cryo und Paraffin)

·        Immunohistochemistry

·        Immunohistology

·        Immunofluorescence

·        ELISA

·        Hormone Measurements

·        DNA Isolation (from cells and tissue)

·        RNA Isolation (from cells and tissue)

·        Classic PCR

·        Quantitative PCR (SybrGreen and TaqMan Probe)

·        Transduction and Transfection (Retrovirus)

·        Isolation of cell populations (Treg, DCs, CD4+ cells)

·        Cell culture (cell lines and primary cultures)

·        Proliferation assays

·        Migration assays

·        siRNA transfection

·        Murine models for miscarriage, pre-eclampsia, pseudo-pregnancy

·        In vivo experiments (mouse): i.p., i.v. injections, immunization, cell transfer, blood pressure measurement, measurement of physiological parameters, gene therapy, in vitro fertilization (IVF), hormonal stimulation